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mouse anti traf6 monoclonal antibody  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti traf6 monoclonal antibody
    Antibodies used for Western blot.
    Mouse Anti Traf6 Monoclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1067 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+traf6+monoclonal+antibody/TRAF6+Antibody/pmc11987747-5-0-7
    Average 96 stars, based on 1067 article reviews
    mouse anti traf6 monoclonal antibody - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy"

    Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

    Journal: Animals : an Open Access Journal from MDPI

    doi: 10.3390/ani15070917

    Antibodies used for Western blot.
    Figure Legend Snippet: Antibodies used for Western blot.

    Techniques Used: Western Blot

    Relative expression values of ( A ) TLR2 , ( B ) TLR3 , ( C ) TLR4 , ( D ) TLR5 , ( E ) IRAK1 , ( F ) TRAF6 , and ( G ) MYD88 mRNA in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).
    Figure Legend Snippet: Relative expression values of ( A ) TLR2 , ( B ) TLR3 , ( C ) TLR4 , ( D ) TLR5 , ( E ) IRAK1 , ( F ) TRAF6 , and ( G ) MYD88 mRNA in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Techniques Used: Expressing

    Expression of TLR2, TLR3, TLR4, TLR5, IRAK1, TRAF6, and MyD88 proteins in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).
    Figure Legend Snippet: Expression of TLR2, TLR3, TLR4, TLR5, IRAK1, TRAF6, and MyD88 proteins in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Techniques Used: Expressing

    Related Articles

    Incubation:

    Article Title: Pathogenic Functions of Tumor Necrosis Factor Receptor- Associated Factor 6 Signaling Following Traumatic Brain Injury.
    Article Snippet: .. The slices were first blocked with 1% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4◦C with mouse anti-TRAF6 monoclonal antibody (sc-8409, 1:200, Santa Cruz Biotechnology) plus the astrocyte marker rabbit anti-GFAP monoclonal antibody (ab7260, 1:500, abcam), the microglial marker goat anti-IBA-1 monoclonal antibody (ab5076,1:500, abcam), or the neuronal marker rabbit antiNeuN monoclonal antibody (ab177487, 1:500, abcam). .. Slices were then incubated at room temperature with Cy3-conjugated and Alexa 488-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, West Grove, PA, United States) for 2 h. The stained sections were examined with a Nikon Ni-E microscope at 20× magnification.

    Article Title: Immunocolocalization of Interferon Regulatory Factory 5 with Tumor Necrosis Factor Receptor-associated Factor 6 and AKT2 in Human Apical Periodontitis.
    Article Snippet: Introduction: Interferon regulatory factor 5 (IRF5) is critical for the regulation of immune and inflammatory responses in health and diseases.. However, the presence of IRF5 in human apical periodontitis remains unknown.. This study aimed to explore the expression and colocalization of IRF5 with tumor necrosis factor receptor–associated factor 6 (TRAF6) and AKT2 in human apical periodontitis.

    Article Title: Early pregnancy affects expression of Toll-like receptor signaling members in ovine spleen
    Article Snippet: The proteins (10 μg/lane) were separated by electrophoresis on 12% SDS-PAGE gels, and were transferred onto polyvinylidene fluoride membranes (Millipore, Bedford, MA, USA). .. The membranes were blocked with 5% fat-free milk at 4 °C overnight, and then incubated with a rabbit anti-TLR2 polyclonal antibody (Abcam, Cambridge, UK, ab191458, 1:1000), a mouse anti-TLR3 monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-32232, 1:1000), a mouse anti-TLR4 monoclonal antibody (Santa Cruz Biotechnology, sc-293072, 1:1000), a mouse anti-TLR5 monoclonal antibody (Santa Cruz Biotechnology, sc-517439, 1:1000), a rabbit anti-TLR7 polyclonal antibody (Abcam, ab113524, 1:1000), a mouse anti-TLR9 monoclonal antibody (Santa Cruz Biotechnology, sc-52966, 1:1000), a mouse anti-MyD88 monoclonal antibody (Santa Cruz Biotechnology, sc-136970, 1:1000), a mouse anti-TRAF6 monoclonal antibody (Santa Cruz Biotechnology, sc-8409, 1:1000) and a rabbit anti-IRAK1 polyclonal antibody (Abcam, ab137327, 1:1000), respectively. .. A horseradish peroxidase (HRP) conjugated goat anti-mouse (Biosharp, BL001A) or anti-rabbit IgG (Biosharp, BL003) was used at a 1:10,000 dilution.

    Article Title: Pathogenic Functions of Tumor Necrosis Factor Receptor- Associated Factor 6 Signaling Following Traumatic Brain Injury
    Article Snippet: .. The slices were first blocked with 1% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4°C with mouse anti-TRAF6 monoclonal antibody (sc-8409, 1:200, Santa Cruz Biotechnology) plus the astrocyte marker rabbit anti-GFAP monoclonal antibody (ab7260, 1:500, abcam), the microglial marker goat anti-IBA-1 monoclonal antibody (ab5076,1:500, abcam), or the neuronal marker rabbit anti-NeuN monoclonal antibody (ab177487, 1:500, abcam). .. Slices were then incubated at room temperature with Cy3-conjugated and Alexa 488-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, West Grove, PA, United States) for 2 h. The stained sections were examined with a Nikon Ni-E microscope at 20× magnification.

    Article Title: Toll-like receptor signaling is changed in ovine lymph node during early pregnancy.
    Article Snippet: The maternal immune system is normally tolerant to the fetus and placenta during pregnancy, which is described as maternal immune tolerance (Williams, 2012).. Interferontau (IFNT) secreted by embryonic trophoblasts is the first pregnancy recognition signal in ruminants and necessary for prostaglandin secretion of endometrial glands and the functions of ovarian corpus luteum (CL), immunological tolerance, implantation, and placentation in ruminants (Bai et al., 2012; Bazer et al., 2018).. IFNT is detectable in uterine flushings and uterine vein serum during early pregnancy and induces expression of IFNstimulated gene (ISG) mRNAs in endometrium and extrauterine tissues, including CL and liver, through paracrine manner and endocrine manner in sheep (Bott et al., 2010; Hansen et al., 2017; Romero et al., 2015).

    Article Title: Pregnancy-induced changes in the toll-like receptor pathway in the ovine duodenum.
    Article Snippet: Toll-like receptor (TLR) signaling plays a key role in the intestinal innate and adaptive immune responses, and pregnancy has effects on intestinal length and villus height.. Nevertheless, it is unclear if early pregnancy modulates the expression of TLR members in the duodenum of ewes.. In this study, ovine duodenums were obtained at day 16 of the estrous cycle (N16), and days 13, 16, and 25 of gestation (P13, P16, and P25) from ewes, and mRNA and protein expression of TLR members were analyzed.

    Article Title: Early pregnancy affects expression of Toll-like receptor signaling members in ovine spleen
    Article Snippet: .. The membranes were blocked with 5% fat-free milk at 4 °C overnight, and then incubated with a rabbit antiTLR2 polyclonal antibody (Abcam, Cambridge, UK, ab191458, 1:1000), a mouse anti-TLR3 monoclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA, sc-32232, 1:1000), a mouse anti-TLR4 monoclonal antibody (Santa Cruz Biotechnology, sc-293072, 1:1000), a mouse anti-TLR5 monoclonal antibody (Santa Cruz Biotechnology, sc-517439, 1:1000), a rabbit anti-TLR7 polyclonal antibody (Abcam, ab113524, 1:1000), a mouse anti-TLR9 monoclonal antibody (Santa Cruz Biotechnology, sc-52966, 1:1000), a mouse anti-MyD88 monoclonal antibody (Santa Cruz Biotechnology, sc-136970, 1:1000), a mouse anti-TRAF6 monoclonal antibody (Santa Cruz Biotechnology, sc-8409, 1:1000) and a rabbit anti-IRAK1 polyclonal antibody (Abcam, ab137327, 1:1000), respectively. .. A horseradish peroxidase (HRP) conjugated goat anti-mouse (Biosharp, BL001A) or anti-rabbit IgG (Biosharp, BL003) was used at a 1:10,000 dilution.

    Marker:

    Article Title: Pathogenic Functions of Tumor Necrosis Factor Receptor- Associated Factor 6 Signaling Following Traumatic Brain Injury.
    Article Snippet: .. The slices were first blocked with 1% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4◦C with mouse anti-TRAF6 monoclonal antibody (sc-8409, 1:200, Santa Cruz Biotechnology) plus the astrocyte marker rabbit anti-GFAP monoclonal antibody (ab7260, 1:500, abcam), the microglial marker goat anti-IBA-1 monoclonal antibody (ab5076,1:500, abcam), or the neuronal marker rabbit antiNeuN monoclonal antibody (ab177487, 1:500, abcam). .. Slices were then incubated at room temperature with Cy3-conjugated and Alexa 488-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, West Grove, PA, United States) for 2 h. The stained sections were examined with a Nikon Ni-E microscope at 20× magnification.

    Article Title: Pathogenic Functions of Tumor Necrosis Factor Receptor- Associated Factor 6 Signaling Following Traumatic Brain Injury
    Article Snippet: .. The slices were first blocked with 1% bovine serum albumin (BSA) for 2 h at room temperature and incubated overnight at 4°C with mouse anti-TRAF6 monoclonal antibody (sc-8409, 1:200, Santa Cruz Biotechnology) plus the astrocyte marker rabbit anti-GFAP monoclonal antibody (ab7260, 1:500, abcam), the microglial marker goat anti-IBA-1 monoclonal antibody (ab5076,1:500, abcam), or the neuronal marker rabbit anti-NeuN monoclonal antibody (ab177487, 1:500, abcam). .. Slices were then incubated at room temperature with Cy3-conjugated and Alexa 488-conjugated secondary antibodies (1:1000, Jackson ImmunoResearch, West Grove, PA, United States) for 2 h. The stained sections were examined with a Nikon Ni-E microscope at 20× magnification.

    other:

    Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy
    Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.



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    Santa Cruz Biotechnology mouse anti traf6 monoclonal antibody
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    Image Search Results


    Antibodies used for Western blot.

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

    doi: 10.3390/ani15070917

    Figure Lengend Snippet: Antibodies used for Western blot.

    Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

    Techniques: Western Blot

    Relative expression values of ( A ) TLR2 , ( B ) TLR3 , ( C ) TLR4 , ( D ) TLR5 , ( E ) IRAK1 , ( F ) TRAF6 , and ( G ) MYD88 mRNA in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

    doi: 10.3390/ani15070917

    Figure Lengend Snippet: Relative expression values of ( A ) TLR2 , ( B ) TLR3 , ( C ) TLR4 , ( D ) TLR5 , ( E ) IRAK1 , ( F ) TRAF6 , and ( G ) MYD88 mRNA in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

    Techniques: Expressing

    Expression of TLR2, TLR3, TLR4, TLR5, IRAK1, TRAF6, and MyD88 proteins in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Journal: Animals : an Open Access Journal from MDPI

    Article Title: Modulation of the Toll-like Receptor Pathway in Ovine Endometria During Early Pregnancy

    doi: 10.3390/ani15070917

    Figure Lengend Snippet: Expression of TLR2, TLR3, TLR4, TLR5, IRAK1, TRAF6, and MyD88 proteins in the endometrium. Significant differences ( p < 0.05) are indicated by different letters (a, b, c, and d).

    Article Snippet: Mouse anti-TRAF6 monoclonal antibody , sc-8409 , Santa Cruz Biotechnology, Santa Cruz, CA, USA , 0.2 μg/mL.

    Techniques: Expressing